| Property |
Value |
| Working Groups |
RG Gerber |
| Subproject |
TP2 |
| Open Access |
Yes |
| Publication Type |
Preprint |
| Peer Reviewed |
No |
| DOI |
10.1101/2021.01.28.428398 |
| Publication Year |
2021 |
| Title |
A quick and versatile protocol for the 3D visualization of transgene expression across the whole body of larval Drosophila |
| Journal |
bioRxiv |
| eISSN |
2692-8205 |
| URL |
http://dx.doi.org/10.1101/2021.01.28.428398 |
| Journal Abbreviation |
bioRxiv |
| Extra |
Final article published in Journal of Neurogenetics here: doi:10.1080/01677063.2021.1892096 |
| Authors |
Kobler O, Weiglein A, Hartung K, Chen Y, Gerber B, Thomas U |
| First Author |
Kobler O |
| Last Author |
Thomas U |
External Resources
2/113073http://resolver.sub.uni-goettingen.de/purl?2/113073 GRO.publications identifier
7227https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=7227 NCBI taxonomy (Drosophila melanogaster)
0000-0001-7173-9919https://orcid.org/0000-0001-7173-9919 ORCID identifier (Oliver Kobler)
0000-0003-4626-328Xhttps://orcid.org/0000-0003-4626-328X ORCID identifier (Alice Weiglein)
0000-0002-9187-930Xhttps://orcid.org/0000-0002-9187-930X ORCID identifier (Yi-chun Chen)
0000-0003-3003-0051https://orcid.org/0000-0003-3003-0051 ORCID identifier (Bertram Gerber)
0000-0002-4372-8611https://orcid.org/0000-0002-4372-8611 ORCID identifier (Ulrich Thomas)
01zwmgk08https://ror.org/01zwmgk08 ROR identifier (01zwmgk08, Leibniz Institute for Neurobiology)
00ggpsq73https://ror.org/00ggpsq73 ROR identifier (00ggpsq73, Otto-von-Guericke University Magdeburg)
biorxiv....-1.pdfhttps://www.biorxiv.org/content/biorxiv/early/2021/01/28/2021.01.28.428398/DC1/embed/media-1.pdf Supplemental Material (Figures S1-S3)
biorxiv....-2.pdfhttps://www.biorxiv.org/content/biorxiv/early/2021/01/28/2021.01.28.428398/DC2/embed/media-2.pdf Supplemental Material (Table 1)
biorxiv....-3.mp4https://www.biorxiv.org/content/biorxiv/early/2021/01/28/2021.01.28.428398/DC3/embed/media-3.mp4 Supplemental Material (Movie 1)
biorxiv....-4.mp4https://www.biorxiv.org/content/biorxiv/early/2021/01/28/2021.01.28.428398/DC4/embed/media-4.mp4 Supplemental Material (Movie 2)
biorxiv....-5.mp4https://www.biorxiv.org/content/biorxiv/early/2021/01/28/2021.01.28.428398/DC5/embed/media-5.mp4 Supplemental Material (Movie 3)
biorxiv....-6.mp4https://www.biorxiv.org/content/biorxiv/early/2021/01/28/2021.01.28.428398/DC6/embed/media-6.mp4 Supplemental Material (Movie 4)
biorxiv....-7.mp4https://www.biorxiv.org/content/biorxiv/early/2021/01/28/2021.01.28.428398/DC7/embed/media-7.mp4 Supplemental Material (Movie 5)
biorxiv....-8.mp4https://www.biorxiv.org/content/biorxiv/early/2021/01/28/2021.01.28.428398/DC8/embed/media-8.mp4 Supplemental Material (Movie 6)
biorxiv....-9.pdfhttps://www.biorxiv.org/content/biorxiv/early/2021/01/28/2021.01.28.428398/DC9/embed/media-9.pdf Supplemental Material (Movie Legends)
42https://for2705.de/literature/publications/42 Published Data Registry entry (FOR 2705, final article)
SCR_007370https://scicrunch.org/resolver/SCR_007370 SciCrunch identifier (RRID:SCR_007370, Imaris)
SCR_021315https://scicrunch.org/resolver/SCR_021315 SciCrunch identifier (RRID:SCR_021315, Adobe Premiere Pro)
SCR_002285https://scicrunch.org/resolver/SCR_002285 SciCrunch identifier (RRID:SCR_002285, Fiji)
Linked Fly Strain
| Genotype |
Category |
FlyBase ID |
Genetic Description |
|
| SS01671-Gal4 |
Binary expression systems (GAL4/UAS, lexA/lexAop, QF/QS, TALE/VAS system etc.) |
|
AD: R21D02 / DBD: R55D08 |
|
| w1118; MB247:mCherry-CAAX |
Fluorescent protein, lacZ and other reporters/tools |
|
MB247:mCherry-CAAX |
|
| w[*] P{w[+mC]=PTT-GC}dlg1[YC0005] |
Fluorescent protein, lacZ and other reporters/tools |
FBst0050859 |
dlg1 |
|
| w[*]; PBac{y[+mDint2] w[+mC]=UAS-mIFP-T2A-HO1}VK00005 |
Binary expression systems (GAL4/UAS, lexA/lexAop, QF/QS, TALE/VAS system etc.) |
FBst0064181 |
UAS-mIFP-T2A-HO1 |
|
| w[*]; P{w[+mC]=UAS-Ir76b.Z}2/CyO; TM2/TM6B, Tb[1] |
Binary expression systems (GAL4/UAS, lexA/lexAop, QF/QS, TALE/VAS system etc.) |
FBst0052610 |
UAS-Ir76b |
|
| w[*]; P{w[+mC]=UAS-mCherry.CAAX.S}2 |
Binary expression systems (GAL4/UAS, lexA/lexAop, QF/QS, TALE/VAS system etc.) |
FBst0059021 |
UAS-mCherry |
|
| w[1118]; PBac{681.P.FSVS-1}PMCA[CPTI001995] |
Fluorescent protein, lacZ and other reporters/tools |
FBst0325248 |
PBac{681.P.FSVS-1}PMCA[CPTI001995] |
|
| w[1118]; P{w[+mW.hs]=GawB}VGlut[OK371] |
Binary expression systems (GAL4/UAS, lexA/lexAop, QF/QS, TALE/VAS system etc.) |
FBst0026160 |
VGlut-GAL4 |
|
| y1 w*;MB247:mCherry-CAAX, UAS-mIFP-T2A-HO1 |
- Not set - |
|
MB247:mCherry-CAAX, UAS-mIFP-T2A-HO1 |
|
| y[1] w[*]; P{w[+m*]=nSyb-GAL4.S}3 |
Binary expression systems (GAL4/UAS, lexA/lexAop, QF/QS, TALE/VAS system etc.) |
FBst0051635 |
nSyb-GAL4 |
|